Kingston General Hospital
Watkins C, 5-4-321
Kingston, Ontario K7L 3Y7
Dr. Susan Brogly is an epidemiologist whose research interests include perinatal epidemiology, addiction, surgical outcomes, and the application of innovative methodologies in medical studies. Dr. Brogly has published over 40 scientific articles, served on grant review boards for the Canadian Institutes of Health Research, the Centers for Disease Control, and the March of Dimes, and is a recipient of the Boston University School of Public Health Excellence in Teaching Award and the Randall Coates New Investigator Memorial Award. Dr. Brogly’s research has been supported with grants from the US National Institute of Child Health and Human Development, the American Cancer Society, Queen’s University, and other organizations. Dr. Brogly joined Queen’s Department of Surgery in 2014 after spending 12 years in Boston where she held appointments as a faculty member of the Department of Epidemiology at the Boston University School of Public Health (2009-2014) and a research faculty member at the Center for Biostatistics in AIDS Research at the Harvard School of Public Health (2002-2009). Dr. Brogly teaches in Queen’s Public Health Sciences Department and the Department of Epidemiology at the Boston University School of Public Health. Dr. Brogly received her Ph.D. and M.Sc. in epidemiology from McGill University, and a B.Sc. in chemical engineering from Queen’s University.
Publications: Dr. Brogly’s publications are available on PubMed https://www.ncbi.nlm.nih.gov/pubmed/?term=brogly+s
Click here to read Dr. Brogly's article in the Queen's Gazette April 10, 2017 entitled
"Addressing Addictions" Study reveals some women with prenatal opioid dependence are not being treated.
Dr Mark Ormiston
Botterell Hall A209,
Biomedical And Molecular Sciences
My research is centered on the cellular mechanisms governing vascular remodeling in health and disease, with a particular focus on the capacity of circulating cells of the immune system to regulate blood vessel integrity, growth and repair. This interest is rooted in my study of pulmonary arterial hypertension (PAH), a disease of pathological vascular remodeling and right heart failure that is linked to immune dysfunction. PAH is strongly associated with loss-of-function mutations in the bone morphogenetic protein (BMP) type II receptor (BMPR-II), a receptor of the transforming growth factor-β (TGFβ) superfamily. Identification of this link between PAH and BMPR-II haploinsufficiency has provided considerable insight into the mechanisms governing pulmonary vascular homeostasis and makes PAH an ideal candidate for proof-of-concept regenerative and cell-based therapeutic strategies.
More specifically, I am interested in the capacity of innate lymphocytes, such as Natural Killer (NK) cells, to influence vascular growth and repair. Beyond their traditional role as the cytotoxic effector cells of innate immunity, there is a growing body of literature supporting a role for NK cells in the regulation of vascular remodelling, particularly in pregnancy. My previous work has identified a phenotypic and functional defect in the circulating NK cells of PAH patients, associated with increased TGFβ signalling and elevated production of matrix metalloproteinase (MMP)-9 by these cells. Through my ongoing studies, I aim to (i) determine the mechanisms by which NK cells influence pulmonary vascular homeostasis in health and disease and (ii) clarify the impact of BMPR-II mutations on these processes.
EMPLOYMENT AND EDUCATION
October 2015 - 2020
Queen’s University, Kingston, ON Canada
Funding: Tier II Canada Research Chair, Canadian Institutes of Health Research
Tier II Canada Research Chair in Regenerative Cardiovascular Medicine, Canadian Institutes of Health Research. 2015-2020. $500,000.
Project Grant, Canadian Institutes of Health Research. “Cellular and molecular mechanisms of Natural Killer cell-mediated vascular remodeling in pulmonary arterial hypertension.”
2017-2022. $830,025. Role: Principal Investigator.
2016-2017. $100,000 bridge grant. Role: Principal Investigator.
William M. Spear Endowment Fund in Pulmonary Research/ Richard K. Start Memorial Fund, Queen’s University Faculty of Health Sciences. “Immunophenotyping of natural killer cells from pulmonary arterial hypertension patients using CyTOF mass cytometry.”
Role: Principal Investigator
John Alexander Stewart Postdoctoral Fellowship (Salary award for Dr. M. Ratsep), Queen’s University Department of Medicine. “Interleukin-15 signalling by the pulmonary endothelium, and its role in regulating Natural Killer cell homeostasis and pathogenesis of pulmonary arterial hypertension.”
Seed Grant – Research Team Project, Canadian Vascular Network. “A central role for hsa-miR-138-5p and the Mitochondrial Calcium Uniporter (MCU) in human and experimental PAH.”
Role: Co-investigator (PI: Dr. Stephen Archer)
Research Leader’s Fund, Queen’s University. “Generation of blood outgrowth endothelial cells for the study of immune dysfunction in pulmonary arterial hypertension.”
Role: Principal Investigator
John R. Evans Leaders Fund, Canadian Foundation for Innovation.
Small Infrastructure Fund, “Regenerative Cardiovascular Medicine Laboratory.” Ontario Research Fund.
Role: Principal Investigator
Project Grant, Canadian Institutes of Health Research. “Understanding the Role of von Willebrand Factor in Angiodysplasia.”
Role: Collaborator. (PI: Dr. Paula James)
PREVIOUSLY HELD GRANTS AND AWARDS
National Grant Review/Grant-In-Aid, Ontario Thoracic Society. “Examining the impact of endothelial BMPR2 loss on immune dysfunction in the pathogenesis of pulmonary arterial hypertension.”
Role: Principal Investigator
William M. Spear Endowment Fund in Pulmonary Research/ Richard K. Start Memorial Fund, Queen’s University Faculty of Health Sciences. “Interleukin-15 signaling and pulmonary hypertension in mice bearing an endothelial-specific deletion of bmpr2.”
Role: Principal Investigator
Summer Work Exchange Program (SWEP) Studentship, Queen’s University.
Role: Supervisor - conceived project and wrote proposal
Summer Work Exchange Program (SWEP) Studentship, Ms. Anne Theilmann, Queen’s University.
Supervisor: Conceived project and wrote proposal
PEER-REVIEWED ORIGINAL RESEARCH
Under review/In revision
M. Rätsep, S.D. Moore, S. Jafri, H.J.M. Brady, O. Mandelboim, M. Southwood, N.W. Morrell, F. Colucci, M.L. Ormiston. “Spontaneous pulmonary hypertension in genetic mouse models of natural killer cell deficiency.” Submitted to The American Journal of Physiology, Lung Cellular and Molecular Physiology, September 2017. Reviewed and in revision.
B. Garfield, A. Crosby, D. Shao, P. Yang, R. Paul, S. Sawiak, M.L. Ormiston, S. Moore, L. Parfitt, C. Harries, N.W. Morrell, M. Polkey, S. J. Wort, P. Kemp. “Growth and differentiation factor 15 mediated skeletal muscle loss in pulmonary arterial hypertension is transforming growth factor activated kinase 1 dependent.” Submitted to Thorax, December 2017.
K.H. Chen, A. Dasgupta, J. Lin, F. Potus, S. Bonnet, J. Iremonger, J. Fu, J. Mewburn, D. Wu, K. Dunham-Snary, Z. C. Jing, Anne L. Theilmann, C. Hindmarch, M.L. Ormiston, A. Lawrie, S.L. Archer “Critical role of MiD49 and MiD51 mediated mitotic fission in human vascular disease.” Submitted to Circulation, August 2017. Reviewed and resubmitted.
E. Ferrer, B.J. Dunmore, M.L. Ormiston, S. Moore, J. Deighton, L. Long, X.D. Yang, D.J. Stewart, N.W. Morrell. “Translationally controlled tumor protein export via exosomes is involved in vascular remodeling in pulmonary arterial hypertension.” Submitted to The American Journal of Respiratory Cell and Molecular Biology, July, 2017. Reviewed and resubmitted.
P. Caruso, B.J. Dunmore, K. Schlosser, S. Schoors, C. Dos Santos, C. Perez-Iratxeta, J. Lavoie, L.L. Long, L. Hurst, M.L. Ormiston, A. Hata, P. Carmeliet, D.J. Stewart, N.W. Morrell. “Identification of miR-124a as a major regulator of enhanced endothelial cell glycolysis in pulmonary arterial hypertension by miRNA and proteomic profiling.” Circulation. 2017 Sep 26. [Epub ahead of print].
S. Jafri, S.D. Moore, N.W. Morrell, M.L. Ormiston. “A sex-specific reconstitution bias in the competitive CD45.1/CD45.2 congenic bone marrow transplant model.” Scientific Reports. 2017 Jun 14;7(1):3495.
S.N. Selvam, L.J. Casey, M.L. Bowman, L.G. Hawke, A.J. Longmore, J. Mewburn, M.L. Ormiston, S.L. Archer, D.H. Maurice, P. James. “Abnormal angiogenesis in blood outgrowth endothelial cells derived from von Willebrand disease patients.” Blood Coagulation and Fibrinolysis. 2017 March 30. [Epub ahead of print]
Z. Hong, K.H. Chen, A. DasGupta, F. Potus, K. Dunham-Snary, S. Bonnet, L. Tian, J. Fu, S. Breuils-Bonnet, S. Provencher, D. Wu, J. Mewburn, M.L. Ormiston, S.L. Archer. “miR-138 and miR-25 Downregulate MCU, Causing Pulmonary Arterial Hypertension’s Cancer Phenotype.” The American Journal of Respiratory and Critical Care Medicine. 2017 Feb 15;195(4):515-529
BOOK CHAPTERS AND REVIEWS
T. Thenappan, M.L. Ormiston, J. Ryan, S.L. Archer. “Etiology, Diagnosis and Management of Pulmonary Arterial Hypertension.” Invited review, The British Medical Journal. In press.
S. Jafri and M.L. Ormiston. “Immune regulation of systemic hypertension, pulmonary arterial hypertension and preeclampsia: shared disease mechanisms and translational opportunities.” The American Journal of Physiology, Regulatory, Integrative and Comparative Physiology. 2017 Oct 4 [Epub ahead of print]
PUBLISHED AND OTHER SELECTED ABSTRACTS
A.L. Theilmann, L. Hawke, R.L. Hilton, J. Mackeil, K.J. Dunham-Snary, P.D. James, D.H. Maurice, S.L. Archer, M.L. Ormiston “Sub-haploinsufficiency of BMPR2 causes a shift in the endothelialproliferative and metabolic response to BMP9.” Submitted to the American Thoracic Society International Conference, San Diego, CA. May 2018.
INVITED ORAL PRESENTATIONS
“Innate Lymphoid Cell plasticity in chronic hypoxia and pulmonary vascular disease” 10th Annual Luis G. Melo Memorial Lecture. Queen’s University. November 29, 2017.
“Natural Killer cells in pulmonary vascular disease.” Beckman Coulter CytoFLEX LX tour. University of Ottawa. June 15, 2017.
“Historical perspective on stem cells in PAH.” Pulmonary Vascular Research Institute Annual World Congress. Miami, FL. January 26, 2017.
“Stem Cell Therapies for Cardiovascular Disease: Facts & Fiction.” Queen’s University Department of Medicine 3rd Annual Continuing Medical Education Day. Kingston, Ontario, Canada. May 11, 2016.
“Targeting Endothelial and Immune Dysfunction for the Treatment of Pulmonary Vascular Disease.” Queen’s University Department of Biomedical and Molecular Sciences Plenary Lecture Series. Queen’s University, Kingston, Ontario, Canada. May 3, 2016.
“Examination of cardiopulmonary function in mouse models of Natural Killer cell deficiency.” Stanford University 2nd International Symposium on Immunity and the Pathogenesis of Pulmonary Hypertension. Stanford, CA. November 6, 2015.
First recipient of the Fellowship in Surgical Education
Human Mobility Research Centre
Kingston, ON Kingston General Hospital
K7M 1L2, Canada Kingston, ON K7L 2V7, Canada
Phone: 613-549 6666 ext.7308
• Computer-Assisted Hip Surgery
• Computer-Assisted Fracture Management
• Image-guided Planning for Total Ankle Arthroplasty
• Development of advanced visualization techniques for image-free computer-assisted Anterior Cruciate Ligament Reconstruction
• Novel applications for kinematic reconstructionin Total Knee Arthroplasties
• Development and testing of an intra-operative tracking system for patella movement
• Application for fluoroscopy guided cartilage repair surgery
• Application of Virtual Reality techniques in computer-assisted surgeries for improved man-made interfaces
• Development of application for endoscopy based surgical navigation
• Investigation of surgical navigation for shoulder surgeries
• High level, multi colored Petri Nets: High Level Petri Nets are used for simulation of complex parallel algorithms. In my thesis, I investigated the relationship between structural properties of these nets and behavior of the simulated algorithm